A application of oil degrading bacteria to an oil spill b production by from bio 024233 at long beach city college. Application of chemical dispersants to oil spills in the marine environment is a common practice to disperse oil into the water column and stimulate oil biodegradation by increasing its. A strain of g40 which can remove oil isolated from contaminated soil. Isolation, screening and activity of hydrocarbondegrading bacteria. Although a number of reports have been published on the degradation of petroleum hydrocarbons by marine microorganisms in warm. Study on the biodegradation of crude oil by free and. Isolation and identification of crude oil degrading and. They isolated some crude oil degrading bacteria that belong to genus pseudomonas, mycobacterium, acinetobacter, micrococcus and nocardia. After incubation, the msm 50 ml was mixed with 50 ml chloroform in order to dissolve the crude oil and then dried in the fume hood. Corexit, the chemical dispersant applied in the bp oil spill, was tested against these bacteria for toxicity purposes. Microbial degradation of petroleum hydrocarbon contaminants. This paper presents an updated overview of petroleum. Others, like neptuniibacter, werent known to be involved with degrading oil in the gulf.
Isolation and characterisation of crude oil sludge degrading bacteria. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 11 strains were selected from the 25 isolated strains for further study. Altogen labs has developed active bioremediation technology based on natural oildegrading bacteria for largescale remediation of hydrocarboncontaminated water and soil. This study aims to isolate the crude oil degrading and biosurfactant producing bacteria from the oil contaminated soils of gachsaran, iran. Isolation and characterization of oildegrading bacteria. Adeleke1,3,4 background high demand for petroleum products all over the world has led to an increase in crude. Characterization of oildegrading bacteria from oilcontaminated. The use of microorganisms in remediating environmental contaminants such as crude oil sludge has become a promising technique owing to its economy and the fact it is environmentally friendly. Studies on crude oil degrading bacteria isolated from libyan desert farag m. Chemical dispersants enhance the activity of oil and gas. Diesel fuels are used by different vehicles, diesel. Isolation and characterization of bacteria degrading n. Other heterotrophic bacteria included several species of escherichia, klebsiella, nonoildegrading pseudomonas, vibrio, streptococcus, staphylococcus and bacillus. In the present study, altogether 32 morphologically distinct bacteria were isolated from crude oil contaminated soil and 18 different hydrocarbondegrading bacteria were number.
Isolation was performed in peptonewater medium with yeast extract. The burning of fossil fuels creates energy that runs the entire economy of our planet. In the present study the expression vector of the rpf1 gene from an oildegrading bacterium rhodococcus erythropolis kb1 was constructed and expressed in escherichia coli. Polycyclic aromatic hydrocarbons pahs, as the major components of oil sludge, are hydrophobic and recalcitrant. The bacteria suspension was serially diluted in a normal saline solution then cultured on plates which were then incubated at 37.
Oildegrading bacteria are essential in marine environments that are subject to frequent natural and anthropogenic seepage. Genetic potential of oileating bacteria from the bp oil. The only thing that is new is the quantity of oil being spilt in the sea from oil disasters. Enzyme characterization and biological activities of a. This paper presents an updated overview of petroleum hydrocarbon degradation by microorganisms. Us20330799a1 us490,508 us2012490508a us20330799a1 us 20330799 a1 us20330799 a1 us 20330799a1 us 2012490508 a us2012490508 a us 2012490508a us 20330799 a1 us20330799 a1 us 20330799a1 authority us united states prior art keywords crude oil bacteria strain altogen serratia prior art date 20120607 legal status the legal status is an. Zulfa aldisi1, samir jaoua1, dhabia althani1, saeed. Us20330799a1 crude oil degrading microorganisms and.
A total of twenty bacterial cultures were isolated from hydrocarbon contaminated soil. Another consortium of preisolated oildegrading bacteria in association with three species of plants effectively remediated contaminated siltloam soil more than silt, loam and sandy loam with an average 80% reduction of total petroleum hydrocarbon ghosh and syed 2001. Biodegradation of crude oil using local bacterial isolates iopscience. Screening of hydrocarbon degrading bacteria isolated from. Isolation and characterization of dye degrading bacteria. Floodgate 36 listed 25 genera of hydrocarbon degrading bacteria and 25 genera of hydrocarbon degrading fungi which were isolated.
In fact, they have been around for millions of years. The study revealed a high efficiency of bacteria adapted to the biodegradation of hydrocarbons petroleum isolated from soil contaminated with oil residues. Bushnell and hass broth was used as selective enrichment medium with engine oil as a sole carbon and energy source and 19 different isolates were obtained. Isolation and characterisation of crude oil sludge.
Twentyfive crudeoildegrading bacteria were isolated from oilcontaminated sites in the persian gulf and the caspian sea. Thus, in order to get insight of the bioremediation process of hydrocarbons in oil contaminated environments of ouagadougou city, the present study focused on the isolation, characterization and identification of indigenous hydrocarbondegrading bacterial strains with regards to the conditions for optimizing their activities and. Isolation and screening of hydrocarbon degrading bacteria. Frontiers petroleum hydrocarbondegrading bacteria for. Lutibacterium is a genus of gramnegative staining bacteria. An important way of enhancing the rate of pah desorption is to compost. Isolation of oil degrading bacteria from the deepwater horizon oil spill for biodiesel processing there are a number of problems with fossil fuels in our society. Isolation and identification of oil degrading bacteria. Parametric optimization of media for the crude oil.
By enrichment, domestication, and separation of culture from the soil sample of qianjiang guanghua oilfield, the pure culture of three petroleum degrading bacteria g40, g53, and g94 was identified from the medium supplemented with oil, which served as the sole source of carbon. The modified polyelectrolytemagnetite nanocoating was. Fifteen crude oil degrading bacteria were isolated from oil contaminated sites in the persian gulf at khorramshahr provenance. Isolation, identification and characterization of oil. In this survey 15 crudeoil degrading bacteria were isolated from different area at persian gulf in iran. Isolated bacteria are able to degrade dye either single or consortia methods. Isolation and characterization of crudeoildegrading. Enrichment, isolation and identification of hydrocarbon degrading bacteria.
Oileating bacteria mitigated deepwater horizon oil. Some of the main oildegrading bacteria in the gulf of mexico, were the marinobacter sp. An ethereal solution of crude oil 10% wv was uniformly sprayed over the surface of agar plate. Hydrocarbon degrading microorganisms were isolated from ten oil polluted areas in and around navi mumbai. Alcanivorax borkumensis was the first hcb to have its genome sequenced. Atlas and bartha, 1992 was transferred to each flask and sterilized.
Many indigenous microorganisms in water and soil are capable of degrading hydrocarbon contaminants. Isolation of hydrocarbon degrading bacteria from soils. Most of the problems are caused due to the byproducts, which are created when then fuels are being burned. The most common is a specific fractional distillate of petroleum fuel oil, but alternatives that are not derived from55 petroleum, such as. Isolation and identification of petroleum hydrocarbon. Distribution and biodegradation potential of oildegrading bacteria in. Isolation and characterisation of crude oil sludge degrading bacteria linda u. Isolation and characterization of oildegrading bacteria from bilge wa ter seed culture broth were transferred into a 500 ml erlenme yer flask containing 250 ml of onr7a m edium supplemented.
Despite its toxicity, a considerable fraction of petroleum oil entering marine systems is eliminated by the hydrocarbondegrading activities of microbial communities, in particular by a recently discovered group of specialists, the hydrocarbonoclastic bacteria hcb. Bacteria are the most active agents in petroleum degradation, and they work as primary degraders of spilled oil in environment 37, 38. Not only did the gene sequencing show several bacteria could break down aromatic hydrocarbons, it illuminated how various species worked together to maximize the genetic potential of the whole microbial community. Identification of petroleum degrading bacteria and. Introduction the biodegradation of petroleum and other hydrocarbons in the environment is a.
Extraction and characterization of oil degrading bacteria. Petroleum hydrocarbondegrading bacteria for the remediation of. Isolation and characterization of hydrocarbondegrading bacteria. For several years scientists have pursued genetic modifications to enhance the ability of various types of engineered bacteria to degrade oil spills, whether on land or in sea. Introduction the biodegradation of petroleum and other hydrocarbons in the environment is a complex process, whos quantitative and qualitative aspects depend on the nature and amount of the oil or hydrocarbons present, the ambient and seasonal environmental. With the sharp increase in population and modernization of society, environmental pollution resulting from petroleum hydrocarbons has. Unusual metabolism and hypervariation in the genome of a.
Assessing biodegradation of oil with marine bacteria by monitoring o 2 and co 2 concentrations online in a closed loop introduction in april 2010, the deepwaterhorizon dwh well explosion at the gulf of mexico caused the worst oil. Isolation and characterization of hydrocarbondegrading. In order to improve the degradation efficiency of petroleum degrading bacteria to petroleum hydrocarbon pollutants, we must have an. Dispersants set the stage for oildegrading bacteria. Similarly, in preliminary study 57 oil degrading bacteria were isolated from oil impregnated soil in search of potent hydrocarbon degrader 14. Study of oil degrading bacteria isolated from oil contaminated sites. The strains obtained in this study demonstrated the potential. However, some surfactants, such as corexit 9500, were reported to exhibit adverse impacts on oildegrading bacteria kleindienst et al.
Extraction and characterization of oil degrading bacteria article pdf available in journal of applied sciences 610 october 2006 with 2,247 reads how we measure reads. Elghazawani2 and ahmed issa3 1microbiology laboratory, botany department, omar almukhtar university, elbeida libya 2inspection and corrosion section, sarir. Pdf hydrocarbon degrading microorganisms were isolated from ten oil polluted areas in and around navi mumbai. Isolation of microorganism from natural source for bioremediation of oilspill susant kumar padhi 1. Parametric optimization of media for the crude oil degrading bacteria isolated from crude oil contaminated site preeti singh, dipali parmar and ajit pandya pacific university, udaipur, rajasthan, india corresponding author abstract introduction due to rapid growth of human population, demand for crude oil and its other. The present work was conducted to isolate and identify bacteria from oil 21 contaminated soil to evaluate their role in biodegradation of commercial diesel under laboratory 22 conditions. Thus, we predict that the relevant resources are probably bacterial compounds released by cell lysis e. Isolation, screening and activity of hydrocarbondegrading bacteria from harsh soils. A application of oil degrading bacteria to an oil spill b. The aim of this study is to construct petroleum degrading agent pda which can effectively degrade oil. Of the 20 isolates, ram03, ram06, ram, and ram17 were specifically chosen based on their relatively higher growth on salt medium amended with 4 % crude oil, emulsion index, surface tension, and degradation percentage. Reconstructing metabolic pathways of hydrocarbondegrading. Of the 1 crude oil degrading isolates 15 isolates were selected and cultivated in bh media with 1% crude oil as a sole carbon and energy source.
Microbial products for eor oil degrading microbes can be isolated from oil contaminated soil or water waste. Bacteria enriched from surface and plume waters of the 2010 deepwater horizon oil spill show that the combined capabilities of communitywide hydrocarbon degradation is greater than its individual. Pdf study of oil degrading bacteria isolated from oil. Several bacteria are even known to feed exclusively on hydrocarbons 39. The role of algae in bioremediation of organic pollutants. The objective of the present study is to analyze the physicochemical characterization of textile dye effluents and isolate and characterization of dye degrading bacteria from dye. Oil displacement area, emulsification index and bacterial phylogeny using 16s rrna analysis were studied. Isolation, identification and characterization of oil degrading bacteria isolated 431 biochemical characterization of selected microorganisms. Pdf the present study was conducted to determine the isolation, identification and characterization of efficient oil degrading bacterial strains. Sediments samples were collected once in a month for a period of twelve months from area surrounding ennore creek and screened for hydrocarbon degrading bacteria. Assessing biodegradation of oil with marine bacteria. About 50ml medium was dispensed in 250ml conical flasks. Resuscitationpromoting factors rpf are a class of muralytic enzymes, which participate in recovery of dormant cells and promoting bacteria growth in poor media.
References this alphaproteobacteriarelated article is a stub. Pure colonies of oil sludge degrading bacteria were identified and. This can also be done by adding substances synthesized by microbes which can assist in oil recovery process 9. Introduction rates of biodegradation depend greatly on the composition,petroleum is a liquid mixture consisting of complex hydrocarbons.
Pdf extraction and characterization of oil degrading bacteria. Index terms microorganisms, hydrocarbon, bioremediation i. Characterization of crude oil degrading bacteria isolated. For examining the degradation of oil, bushnell haas medium bhm supplemented with 5g50ml of petrol, diesel and used engine oil to be used 11. Crude oil and petroleum is a complex mixture of hydrocarbons, which cause widespread environmental contaminants following a spill. Biodegradation of tapis crude oil using consortium of. A smart dress for oildegrading bacteria sciencedaily. It includes the hydrocarbondegrading strain lutibacterium anuloederans lc8. The separation of petroleum hydrocarbon degrading bacteria was as follows.
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